Illustration(s) pertain to the topic addressed in this publication, not the specific research or data presented in the publication

Single-cell cytokine profiles in normal humans: comparison of flow cytometric reagents and stimulation protocols


Cytokines are produced and function at a micro environmental level: intracellular assessment has only recently become practically feasible. We used 3-color flow cytometry to examine surface and cytoplasmic antigens on peripheral blood lymphocytes of 18 normal donors, assessing the applicability/comparability of various directly conjugated anti-human cytokine reagents and stimulation protocols using separated cells or whole blood preparations.… Read more